Abstract: Single-cell transcriptome sequencing, often referred to single-cell RNA sequencing (scRNA-seq), is a technique to measure gene expression and analyze the transcriptome sequence at the single-cell level. In recent years, the application of the scRNA-seq technique has been gradually increased, especially in the field of ophthalmology. It can be used to identify cell types and cell subtypes of retina and cornea, facilitate the investigation of the early human retina development, the time course of cell differentiation, and the retinal and retinal organoids formation. In addition, single-cell transcriptome sequencing can be used to study molecular mechanisms of wound healing in the eye and help to found new biological targets and markers of eye diseases, such as age-related macular degeneration and retinoblastoma, providing a new direction for the treatment.(Int Rev Ophthalmol, 2022, 46:  184-188)

Key words: scRNA-seq, single-cell analysis, ophthalmology